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After RNA quantification using the Nanodrop 2000 (Thermo Fisher Scientific, USA), 1 g of total RNA was reverse transcribed in a total volume of 20 l using the PrimeScript RT reagent kit (Takara Bio, Tokyo, Japan)

Analysis of RcGPX promoter sequences showed that diverse cis -acting regulatory elements, such as environmental stress binding sites, were discovered and their quantity differed in each RcGPX promoter (Supplementary Table 5), which implied their potential roles in abiotic stresses