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This increase in oxidative stress was not seen in chronic smokers and chronic vapers, likely because they already have a higher level of oxidative stress from long-term exposure, Middlekauff said

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The tubes were incubated at 37C for 30 min, after which 10 l of proteinase K (20 mg/ml) and 50 l of 10% sodium dodecyl sulfate (SDS) was added to the tube and vortexed briefly

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