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Images were processed using Zen (Carl Zeiss Microscopy, White Plains, NY) and ImageJ software ( For quantitative assessment, images were acquired using fixed exposure settings optimized for each channel in a single data acquisition session

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He was miserable. But then on the first day of shooting, Carrey threatened to quit after he was required to be in the makeup chair for eight hours, with Howard revealing, He was ready to give his $20 million back