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Western blotting Samples were lysed in RIPA buffer containing a protease inhibitor, and the total protein content was quantified using a bicinchoninic acid protein assay kit (Beyotime)
Studies showing that plasma cysteine is significantly lower in children with autistic disorder are dominated by one research group that published three studies (one in children with abnormal methylation or GSH:GSSG) [4648] and their findings have been replicated by another research group [60]
Unlike oral supplements, which must pass through the digestive system and are often poorly absorbed, IV nutrients bypass the gut entirely, ensuring 100% bioavailability and faster therapeutic results